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SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and humans. It was named for the effect it produced on infected green monkey cells, which developed an unusual number of vacuoles. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors in animals, but most often persists as a latent infection. SV40 has been widely studied as a model eukaryotic virus, leading to many early discoveries in eukaryotic DNA replication^[1] and transcription.^[2]

The discovery of SV40 revealed that between 1955 and 1963 around 90% of children and 60% of adults in the U.S. were inoculated with SV40-contaminated polio vaccines.^[3]

Human disease

The hypothesis that SV40 might cause cancer in humans has been a particularly controversial area of research.^[4] Several methods have detected SV40 in a variety of human cancers, although how reliable these detection methods are, and whether SV40 has any role in causing these tumors, remains unclear.^[5] As a result of these uncertainties, academic opinion remains divided, with some arguing that this hypothesis is not supported by the data^[6] and others arguing that some cancers may involve SV40.^[7][8] The US National Cancer Institute announced in 2004 that although SV40 does cause cancer in some animal models, "substantial epidemiological evidence has accumulated to indicate that SV40 likely does not cause cancer in humans".^[9] This announcement was based on two studies.^[10][11] This 2004 announcement is in contrast to a 2002 study performed by The National Academy of Sciences Immunization Safety Review committee that stated, "The committee concludes that the biological evidence is moderate that SV40 exposure could lead to cancer in humans under natural conditions.”^[12]

p53 damage and carcinogenicity

SV40 may act as a co-carcinogen with crocidolite asbestos to cause mesothelioma.^[13][14] The mechanism may involve suppression of the transcriptional properties of tumor suppressor p53 in humans by the SV40 large T antigen and SV40 small T-antigen. Tumor suppressor p53 is responsible for initiating regulated cell death ("apoptosis"), or cell cycle arrest when a cell is damaged. A mutated p53 gene may contribute to uncontrolled cellular proliferation, leading to a tumor.

Polio vaccine contamination

Some vaccines made in the USA between 1955 and 1961 were found to be contaminated with SV40, from the growth medium and from the original seed strain. Population level studies show no evidence of any increase in cancer incidence as a result of exposure,^[15] though SV40 has been extensively studied.^[16] A thirty-five year followup found no excess of the cancers putatively associated with SV40.^[17]

Virology

simian virus 40
Virus classification
(unranked):	Virus
Phylum:	incertae sedis
Class:	incertae sedis
Order:	incertae sedis
Family:	Polyomaviridae
Genus:	Betapolyomavirus
Species:	Macaca mulatta polyomavirus 1
Virus:	simian virus 40

SV40 consists of an unenveloped icosahedral virion with a closed circular dsDNA genome^[18] of 5.2 kb.^[19] The virion adheres to cell surface receptors of MHC class I by the virion glycoprotein VP1. Penetration into the cell is through a caveolin vesicle. Inside the cell nucleus, the cellular RNA polymerase II acts to promote early gene expression. This results in an mRNA that is spliced into two segments. The small and large T antigens result from this. The large T antigen has two functions: 5% goes to the plasma cell membrane and 95% returns to the nucleus. Once in the nucleus the large T antigen binds three viral DNA sites, I, II and III. Binding of sites I and II autoregulates early RNA synthesis. Binding to site II takes place in each cell cycle. Binding site I initiates DNA replication at the origin of replication. Early transcription gives two spliced RNAs that are both 19s. Late transcription gives both a longer 16s, which synthesizes the major viral capsid protein VP1; and the smaller 19s, which gives VP2 and VP3 through leaky scanning. All of the proteins, besides the 5% of large T, return to the nucleus because assembly of the viral particle happens there. Eventual release of the viral particles is cytolytic and results in cell death.^{[citation needed]}

Multiplicity reactivation

SV40 is capable of multiplicity reactivation (MR).^[20][21] MR is the process by which two or more virus genomes containing otherwise lethal damage interact within an infected cell to form a viable virus genome. Yamamato and Shimojo observed MR when SV40 virions were irradiated with UV light and allowed to undergo multiple infection of host cells.^[20] Hall studied MR when SV 40 virions were exposed to the DNA crosslinking agent 4, 5’, 8-trimethylpsoralen.^[21] Under conditions in which only a single virus particle entered each host cell, approximately one DNA cross-link was lethal to the virus and could not be repaired. In contrast, when multiple viral genomes infected a host cell, psoralen-induced DNA cross-links were repaired; that is, MR occurred. Hall suggested that the virions with cross-linked DNA were repaired by recombinational repair.^[21] Michod et al. reviewed numerous examples of MR in different viruses and suggested that MR is a common form of sexual interaction that provides the advantage of recombinational repair of genome damages.^[22]

Transcription

The early promoter for SV40 contains three elements. The TATA box is located approximately 20 base-pairs upstream from the transcriptional start site. The 21 base-pair repeats contain six GC boxes and are the site that determines the direction of transcription. Also, the 72 base-pair repeats are transcriptional enhancers. When the SP1 protein interacts with the 21 base-pair repeats it binds either the first or the last three GC boxes. Binding the first three initiates early expression, while binding the last three initiates late expression. The function of the 72 base-pair repeats is to enhance the amount of stable RNA and increase the rate of synthesis. This is done by binding (dimerization) with the AP-1 transcription factor to give a primary transcript that is 3' polyadenylated and 5' capped.^{[citation needed]}

Other animals

SV40 is dormant and is asymptomatic in rhesus monkeys. The virus has been found in many macaque populations in the wild, where it rarely causes disease. However, in monkeys that are immunodeficient—due to, for example, infection with simian immunodeficiency virus—SV40 acts much like the human JC and BK polyomaviruses, producing kidney disease and sometimes a demyelinating disease similar to progressive multifocal leukoencephalopathy. In other species, particularly hamsters, SV40 causes a variety of tumors, generally sarcomas. In rats, the oncogenic SV40 large T antigen was used to establish a brain tumor model for primitive neuroectodermal tumor and medulloblastoma.^[23]

The molecular mechanisms by which the virus reproduces and alters cell function were previously unknown, and research into SV40 vastly increased biologists' understanding of gene expression and the regulation of cell growth.^{[citation needed]}

History

SV40 was first identified by Ben Sweet and Maurice Hilleman in 1960 when they found that between 10-30% of polio vaccines in the USA were contaminated with SV40.^[24] In 1962, Bernice Eddy described the SV40 oncogenic function inducing sarcoma and ependymomas in hamsters inoculated with monkeys cells infected with SV40.^[25] The complete viral genome was sequenced by Fiers and his team at the University of Ghent (Belgium) in 1978.^[26]

See also

• Mason-Pfizer monkey virus packaging signal


• SV40 Cancer Foundation

References

External links

CDC FAQ

• 
  Frequently Asked Questions about Cancer, Simian Virus 40 (SV40), and Polio Vaccine, Science Coordination and Innovation, United States Centers for Disease Control
  

NIH 1997 Conference on SV40

• 
  Simian Virus 40 (SV40:) A Possible Human Polyomavirus Workshop Monday January 27, 1997, Morning Session, transcript of 1997 National Institutes of Health conference on SV40 in humans, (part 1 of 3), United States Food and Drug Administration (FDA)


• 
  Simian Virus 40 (SV40:) A Possible Human Polyomavirus Workshop Monday January 27, 1997 Afternoon Session, transcript of 1997 National Institutes of Health conference on SV40 in humans (part 2 of 3), United States Food and Drug Administration (FDA)


• 
  Simian Virus 40 (SV40:) A Possible Human Polyomavirus Workshop, Tuesday, 28 January 1997, transcript of 1997 National Institutes of Health conference on SV40 in humans (part 3 of 3 ), United States Food and Drug Administration (FDA)

Other

• 
  Simian+virus+40 at the US National Library of Medicine Medical Subject Headings (MeSH)


• 
  SV40 entry in the NCBI Taxonomy database
  


• 
  SV40 entry in the NCBI Genome database